FastQCFastQC Report
Mon 27 Feb 2023
SRR3127492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2779284
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322701.1610904103358994No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC41850.15057835039528167No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG39930.14367009632696767No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG39060.14053979370226288No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34210.12308925608178223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98200.085.060311
CGTAGGG7850.078.055512
ACGGGTA3450.075.1403964
TAGGGCA16200.072.155014
AGTAGGG43100.071.848542
TAGAGGG43500.070.537752
AGGGATG37400.069.2338945
ATAGGGC15850.068.990393
TAAGGGA20250.068.89643
AGGGCAT22900.068.540895
ATAGGGA26450.068.2500463
TATAGGG21450.067.6788942
GAATAGG19850.067.2428051
GTAGGGT7700.066.721473
AAGGGCG14750.066.466254
TAGCGGG13350.065.669172
ATAAGGG35650.065.5770652
GTAGGGC15050.065.454523
ATAGAGG17500.065.2224661
ATAGCGG5650.065.112751
Produced by FastQC (version 0.11.5)