FastQCFastQC Report
Mon 27 Feb 2023
SRR3127489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939855
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334681.1384234936757085No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC49540.16851171231234194No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32300.10986936430538241No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103650.086.8585051
CGTAGGG9500.082.854592
AGGGATG48050.073.2529455
AGTAGGG46200.073.249762
GAATAGG16800.071.301751
GTACGGG7250.070.211712
ATAGCGG7350.068.6551
GGTAAGG20100.068.5113451
TAGAGGG52600.067.831892
TAAGGGA26600.067.6870353
CTACGAC10550.067.260665
AGTACGG3850.066.146791
AAGGGAT50900.066.023044
GTAGGGA20950.065.91853
AAGGGTA20250.065.869624
AGAGGGC41650.065.522033
TAACGGG10800.065.46232
AGGGACT38400.065.227795
GAGGGAT42800.064.752624
TAGCGGG17500.064.639342
Produced by FastQC (version 0.11.5)