FastQCFastQC Report
Mon 27 Feb 2023
SRR3127486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2722512
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346801.2738235864525116No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC29360.10784158159817109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111200.086.457561
ACGGGTA2250.075.436834
GGTAAGG18000.072.33361
CGTAGGG6400.071.458712
AGGGATG40200.071.074015
TAGGGCA14100.070.889224
GTACGGG6200.069.961582
ATAGGGA22000.069.0075453
ATAGCGG5950.068.977241
TAGAGGG45700.068.7102362
AGGGCAT20450.068.478825
AAGAGGG97750.066.0795752
AATAGGG43150.065.887032
TATAGGG23550.065.4666752
ATAGGGC15150.065.353683
ATAAGGG38300.065.2439962
TAAGGGA23500.065.0040743
TAGGGAT16250.064.991734
GAATAGG19750.064.968881
TAGCGGG17150.064.8800662
Produced by FastQC (version 0.11.5)