FastQCFastQC Report
Mon 27 Feb 2023
SRR3127482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067031
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381031.2423415348589564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT122750.087.37741
ATAGGGC17300.073.347393
TAGGGCA15700.073.338744
ACGGGTA2850.072.5562444
TAGAGGG56700.069.501962
AGAGGGC45000.068.510693
AGGGATG42700.068.238715
AATAGGG43950.067.943972
TAGGGTA9700.067.830234
TAAGGGA25700.066.929093
TAGCGGG17500.066.910982
TACGGGT2600.066.879863
GTACGGG7750.066.746282
AGTAGGG48150.066.5099952
AGGGCAT29200.066.149415
ATAGAGG22800.066.052831
AGGGAAT30800.065.917395
AAGAGGG115100.065.778912
GATAGGG46400.065.77532
ATAAGGG45850.065.641232
Produced by FastQC (version 0.11.5)