FastQCFastQC Report
Mon 27 Feb 2023
SRR3127480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063529
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255601.2386547511568773No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25230.12226627297217534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81100.086.7795941
CGTAGGG6100.079.59422
TAGGGCA11250.073.326264
TAGACGG3800.073.238271
GGTAAGG13600.072.836671
ATAGGGA16500.071.421683
ACGGGTA2250.071.2312244
ACGGGAT5600.070.707474
ATAGGGC13950.069.609273
AGTAGGG34950.068.7855152
AGACGGG14700.068.6231162
TATAGGG15850.067.212982
AGGGATG30100.066.977095
TAGAGGG37550.066.910042
AGTAAGG14050.066.810761
AAGAGGG74450.066.6711042
ATAGAGG14050.066.13931
AGAGGGC31750.065.91943
GTACGGG5550.065.399092
TAGGGTA7000.065.320234
Produced by FastQC (version 0.11.5)