FastQCFastQC Report
Mon 27 Feb 2023
SRR3127479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104879
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248691.1814930929521363No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25400.1206720196267814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83250.085.8245541
CGTAGGG5700.075.07372
ATAGGGC13000.073.386473
ACGGGAT5450.070.709974
TAGGGCA13300.070.317714
TAGAGGG37900.070.102012
TAAGGGA19350.069.947933
AGTAAGG12900.069.689561
AGTAGGG36850.068.526422
TATAGGG18100.067.288752
AGGGCAT18750.067.1733255
GGTAAGG13350.066.98791
GAATAGG12400.066.805941
AAGAGGG75450.066.500732
TAACGGG8050.066.009112
ATAGGGA16400.065.909393
AAGGGTA12200.065.87184
AGGGATG33600.065.179345
AAGGGGC61450.065.159994
ATAGAGG15750.065.1478351
Produced by FastQC (version 0.11.5)