FastQCFastQC Report
Mon 27 Feb 2023
SRR3127477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2286215
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260571.1397440748136112No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25980.11363760626187824No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24820.10856371776057808No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTG22880.10007807664633467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79150.085.2533341
AGTAGGG34650.074.000952
ATAGGGA18250.071.799863
TAACGGG7950.071.146862
TAGGGCA12550.070.983884
CGAAGGG24150.070.848582
ATAGGGC14500.070.5396653
ATAGAGG18500.070.094451
TATAGGG15750.069.1310352
AGGGATG38050.069.039185
AGGGCAT20600.068.437245
ATGCGGG12800.068.124512
TAGAGGG41250.067.645582
CGAGGGA14750.067.426733
TAGGGAT13950.066.9014
TAAGGGA17500.066.796743
AAGGGTA14750.066.7876054
GATAGGG33000.066.702882
TAGACGG3400.066.570991
GAATAGG13550.065.772631
Produced by FastQC (version 0.11.5)