FastQCFastQC Report
Mon 27 Feb 2023
SRR3127475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463268
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291491.1833466760417461No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44540.18081670366358837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98950.084.167281
AGGGATG46250.072.159445
CGTAGGG7000.070.5079042
TAGGGCA14350.070.098464
GAGGGAT32050.069.66464
TAGAGGG40900.069.071312
ATAGCGG6300.068.673291
ATAGGGC14450.067.661563
ATAGGGA22350.067.510923
AGTAGGG38350.067.290462
TATAGGG18150.066.299442
AAGAGGG92200.066.276412
AGGGAAT27750.065.722685
GGTAAGG15600.065.71621
GACCGAT1300.065.084218
GAATAGG14500.064.8638151
ATGCGGG12000.064.632252
TAAGGGA22850.064.593673
TAGGGTA7800.064.481594
ATAAGGG41150.063.9682852
Produced by FastQC (version 0.11.5)