FastQCFastQC Report
Mon 27 Feb 2023
SRR3127474.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127474.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156414
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246561.1433797035263173No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38000.1762184812378328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75450.084.089371
AGGGATG40200.077.269655
CGTAGGG6650.073.0249562
ATAGGGA18150.071.175363
GAGGGAT28200.070.720774
AGGGAAT22750.070.0247965
ATAGCGG4650.069.996161
AGTAGGG33600.068.756312
TAGAGGG35900.067.371892
ATAAGGG31900.066.951962
GGTAAGG14400.066.826041
AAGAGGG81850.066.645442
ATAGGGC12600.066.604763
TAGGGCA10850.066.0495154
GGATGGC35950.065.7510457
CGAAGGG22750.065.69522
TAGCGGG13250.065.472322
GTAGGGT7750.064.485183
AGGGCAT17650.064.432395
GTAGGGA17500.064.120173
Produced by FastQC (version 0.11.5)