FastQCFastQC Report
Mon 27 Feb 2023
SRR3127473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188008
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238291.0890728004650807No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC38220.174679434444481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77000.084.034261
AGGGATG40550.074.28755
GACCGAT1300.072.2994848
GAGGGAT29300.071.534544
AGTAGGG35450.070.966622
AGGGCAT18100.069.3236165
ATAGAGG16800.069.188211
TAGAGGG36450.069.1486742
CGTAGGG6500.068.726922
TATAGGG16750.068.219422
ATAGGGA17600.068.088863
AAGGGTA15500.067.914874
ATAGCGG5950.067.227411
TAGGGCA14400.066.902134
ATGCGGG11300.066.166062
GAATAGG13050.065.991041
ATAAGGG31500.065.833152
TAAGGGA18000.065.792533
AGGGAAT21950.065.72835
AGAGGGC30950.065.443293
Produced by FastQC (version 0.11.5)