FastQCFastQC Report
Mon 27 Feb 2023
SRR3127471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196114
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254201.157499109791204No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC60740.27657944897213893No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG26830.12217034270534226No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC26380.12012126875016506No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG25530.11625079572371927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81950.086.223791
CGTAGGG6550.077.678192
TAGGGCA11900.076.010044
AGGGATG42500.074.748985
CTACGAC10600.073.595245
ATAACGG2200.072.850191
GGTAAGG13150.071.334861
TCCGCTA11100.070.9198761
ACGGGTA1800.070.665584
AGGGAAT28400.070.491825
GGATGGC38850.069.9172447
ATGCGGG11150.069.714922
ATAGCGG4550.069.412531
TAGAGGG40050.069.401072
TAAGGGA20100.069.3764953
AGAGGGC29600.069.233163
ATAGGGA23150.069.19023
TAGCGGG10600.068.887832
AAGGGCA38750.068.568414
GAGGGAT32050.068.350484
Produced by FastQC (version 0.11.5)