FastQCFastQC Report
Mon 27 Feb 2023
SRR3127470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251344
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245241.0893048774420968No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61740.27423618958275586No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC28670.12734615411949485No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG27500.12214925839853882No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG27120.12046137773703174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76000.086.083031
AGGGATG46300.074.608045
TAGAGGG39300.072.052642
CGTAGGG7400.071.192182
TAAGGGA20100.071.0815353
CGAAGGG24850.070.982432
TAGGGCA13650.070.583064
ATAGGGA24850.070.1660163
AAGGGCA35600.070.100944
AGAGGGC32050.069.360533
GAGGGAT34300.069.195294
TATAGGG19300.068.972362
GGATGGC40450.068.89947
ATAGCGG5000.068.71781
AGTACGG3050.067.899911
AGGGCAT22500.067.885995
AGTAGGG38000.067.709512
ACGGGTA2500.067.67714
CTACGAC13200.067.648625
TACGACC13200.067.648626
Produced by FastQC (version 0.11.5)