FastQCFastQC Report
Mon 27 Feb 2023
SRR3127469.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127469.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377394
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318411.3393236459753832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92500.085.4550551
ATAGGGC14200.073.822923
AGGGCAT21950.072.814715
ATAGAGG17750.071.263261
GGTAAGG15100.070.6904451
AGTAGGG41750.070.371742
CGTAGGG6350.069.587122
TAGGGTA7800.069.3071444
TAGAGGG45500.069.117732
TAGGGCA13400.069.1092454
GTACGGG4850.068.816312
ATGCGGG11750.068.812182
TAAGGGA18200.068.704473
AAGGGCA37000.068.479694
GAATAGG13750.068.397321
ATAGGGA22000.068.375743
GTAGGGA18150.068.375743
AGGGTAC10250.068.334045
CGAAGGG27900.068.237892
AGTAAGG14900.067.221031
Produced by FastQC (version 0.11.5)