FastQCFastQC Report
Mon 27 Feb 2023
SRR3127468.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127468.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072093
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253971.2256689250916826No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC26690.12880695991927005No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG25290.12205050642031993No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG23880.11524579253923448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79550.085.371951
GGTAAGG10600.072.5228041
TAGGGCA10950.071.8857654
ATAGGGC12700.071.258723
AGGGATG29900.070.254715
AGTAGGG33450.070.17362
CGAAGGG22900.069.569962
AGATAGG9900.068.599381
AGGGCAT17950.068.330595
TAAGGGA16500.067.98823
GAATAGG11850.067.6586761
GTAGGGT7950.067.589353
GTAAGGG29500.067.266782
AGTAAGG13350.067.1220551
CGTAGGG5200.067.076282
TAGAGGG35950.066.342552
GAGGGTA10350.066.034094
ATAAGGG29500.065.8287662
AGGGAAT21500.065.79115
GAGGGAT22950.065.721564
Produced by FastQC (version 0.11.5)