FastQCFastQC Report
Mon 27 Feb 2023
SRR3127467.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127467.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112447
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245541.162348688511475No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC27070.1281452268388272No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG25880.12251194941222195No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG25330.11990833379488337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71950.085.86221
TAGGGCA11550.073.241054
CGTAGGG5550.072.0192
ATAGGGC12700.071.7896963
AGTAGGG35250.071.103222
ATAGGGA18550.070.6845253
AGGGATG27850.069.861715
AGGGCAT19850.069.369925
GTAGGGA17100.067.8836063
GAATAGG12500.067.753321
AGGGAAT21750.067.6315165
GGTAAGG12400.067.5408251
CGAAGGG21650.067.332532
AGAGGGC26200.067.086383
ATAAGGG28750.066.569872
AAGAGGG76100.066.488842
AATAGGG29350.066.1702962
CGAGGGA11450.065.261293
GAGGGAT23300.065.149444
AAGGGGC57750.064.8590244