FastQCFastQC Report
Mon 27 Feb 2023
SRR3127465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences533105
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79271.4869491000834731No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC56431.058515677024226No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC21810.4091126513538609No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG21510.40348524211928233No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG20290.38060044456532954No Hit
TAGACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT8920.16732163457480234No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCATG8870.16638373303570592No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGT7890.14800086286941597No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC7840.14706296133031954No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT7670.14387409609739168No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT7450.1397473293253674No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGTTCG6750.12661670777801745No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT6570.12324026223727033No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT6010.11273576499939036No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5730.10748351638045037No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT5640.10579529361007681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG409.094947E-1294.3145451
CTTGCGG156.8091427E-494.314541
CGGGTAT500.093.987245
ATAGCGG1000.089.598811
TAGGGCG900.089.041324
CGTTTTT32000.086.798851
CGTAGGG1200.086.422462
CGCTACG8400.083.055343
GCGTAGG401.0077201E-982.525221
GATGACC5900.082.039718
CGGGAAT1900.081.620515
TAGCGGG2200.081.422812
TAGGGCA3250.081.225024
GCTACGA8600.081.123834
TCCGCTA8950.080.615221
AGGGATG12550.080.5075
TACGACC8750.080.023426
TAGGGTA1650.079.994344
ACGTAGG650.079.804611
CGAAGGG3500.079.4637762
Produced by FastQC (version 0.11.5)