FastQCFastQC Report
Mon 27 Feb 2023
SRR3127464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549911
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75511.3731312885175964No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC57881.0525339554946163No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG23310.42388677440531286No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC22170.4031561470856193No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG21740.39533669993871734No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGT21190.38533508149500556No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10660.19384955019994143No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCATG9840.17893804633840749No Hit
TAGACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT8800.1600258950993888No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC7660.13929526777969525No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT7610.13838602973935782No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT7610.13838602973935782No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCATGCCTGTTCG6750.1227471354455539No Hit
AAAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT6580.11965572610840662No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6170.11219997417763966No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT6020.10947226005662734No Hit
AGAGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT5770.10492606985494017No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5730.10419867942267022No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG5680.10328944138233277No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT5600.10183466051779289No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATG5580.10147096530165792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGG301.3147655E-894.101971
AGTACGG450.094.101961
GCGTAGG353.5652192E-1094.101961
GGTACGC156.9029885E-493.990737
CGTAGGG1050.089.555692
CGTTTTT32300.087.255521
ATAGCGG650.086.863351
AGGGATG12950.086.732765
CGCTACG9550.084.1487663
CGGGAAT1250.082.7118455
CGAAGGG3700.082.596972
GCTACGA9750.082.422644
CTACGAC9850.081.585855
TACGACC9850.081.585856
ACGACCA9800.081.522577
AAGGGAT12450.080.7791754
GTTACGG353.831883E-880.658821
TAACGGG1050.080.600122
CGACCAA9950.080.293598
TCCGCTA10250.079.87191
Produced by FastQC (version 0.11.5)