FastQCFastQC Report
Mon 27 Feb 2023
SRR3127462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093419
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253761.212179692646336No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50970.24347729718704186No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG29930.142971856088055No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG25880.1236255140514154No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25200.12037723933909074No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC24720.11808433954215568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75800.086.198181
CTACGAC9150.074.983485
AGGGATG38950.072.389845
TAAGGGA18800.071.2225953
AGAGGGC28600.071.050733
TACGACC9650.070.611366
TAGGGCA10550.070.60934
CTATGCG605.456968E-1270.489619
GTACGGG4750.070.472882
CGAAGGG20850.069.646922
GGTAAGG13300.069.167251
ATAGCGG5550.068.850891
ACGGGTA2750.068.5779654
AGGGTAC9800.068.5715265
ATAGGGC13450.068.354893
ATGCGGG9200.068.158672
AGTAGGG33000.068.006482
GAGGGAT29000.067.794644
GCTACGA10100.067.686784
AGGGCAT19500.067.4772345
Produced by FastQC (version 0.11.5)