FastQCFastQC Report
Mon 27 Feb 2023
SRR3127461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140372
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244471.1421846295877538No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50960.2380894536089988No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG30360.1418445017968839No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG28170.13161263556054742No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC26450.12357664929273976No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT25090.11722261363912442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77400.085.756521
CTACGAC9750.074.2308045
AGGGATG37650.073.7718355
TAGACGG3000.073.75061
TAAGGGA19600.072.6531753
CGTAGGG6750.071.77062
TAGGGCA10150.071.768484
AGGGCAT17900.071.414095
TACGACC10200.070.955916
GAGGGAT29750.070.613644
ATAGGGC12750.070.034413
ATAGAGG11550.069.287671
TAGGGCG4700.068.995264
AGGGAAT26800.068.566195
GTACGGG4400.068.4132842
ATAGGGA21950.068.300553
AAGAGGG68800.068.2952042
TATAGGG16300.068.0984952
AGAGGGC25650.067.9758453
GAATAGG13800.067.883311
Produced by FastQC (version 0.11.5)