FastQCFastQC Report
Mon 27 Feb 2023
SRR3127460.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127460.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768116
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370411.3381303384684746No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC31970.11549371485877037No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG30020.10844921238849818No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG28770.10393350567678523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107550.085.1151051
ATAGGGC14850.073.438933
AGTAGGG41350.071.846632
TATAGGG26250.071.451012
TAGCGGG16850.071.4175342
GAATAGG14350.070.7706151
CGTAGGG7750.069.7526862
ATGCGGG13450.069.549762
ATAGCGG6250.069.208421
AGGGCAT23100.068.984665
TAGAGGG48350.068.833782
ATAGGGT13050.068.799873
GGAGCTA59600.068.212519
TAGGGCA15650.068.183034
GTATAGG11350.067.521621
ATAGGGA24300.066.932133
ATAACGG2250.066.868041
ATAGAGG17550.066.70731
ATATAGG11550.065.945351
TAAGGGT12100.065.654753
Produced by FastQC (version 0.11.5)