FastQCFastQC Report
Mon 27 Feb 2023
SRR3127459.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127459.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424514
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292191.2051487432120418No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG42610.1757465619913929No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC39280.16201185062243403No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG35330.1457199257253206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT83150.085.865841
CGTAGGG6400.078.79852
ATAGGGC13150.074.909123
GGATAGG16450.071.0955661
TAGAGGG37550.070.917382
TATAGGG21000.070.473282
TAGGGTA6700.070.345994
TAGACGG4450.069.942431
AGGGCAT18500.069.3475955
AGTAGGG38850.068.423022
ATGCGGG9950.068.210872
GGAGCTA44250.068.1808249
ATAGGGA21400.068.054823
TAAGAGG18050.067.9286351
TAGGGAT13700.067.773494
AGTAAGG15750.067.6682051
TAAGGGA18350.067.551323
GATAGGG36300.067.2569662
AGGGAAT24900.067.187915
GAATAGG13200.067.164641
Produced by FastQC (version 0.11.5)