FastQCFastQC Report
Mon 27 Feb 2023
SRR3127456.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127456.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550610
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266571.0451225393141248No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41290.16188284371189637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89350.085.035011
ACGGGTA3450.077.877754
CGTAGGG6650.076.552562
ATAGCGG4550.075.66731
AGGGATG46200.073.846635
ATAGGGA27750.072.1910863
AGGGCAT23700.069.002835
TAGACGG3350.068.983951
TAGGGTC6150.068.2138444
AGTAGGG39950.068.197532
TAGAGGG40150.068.092622
TAGGGTA8550.067.810454
GAGGGAT41100.067.5508964
TATAGGG20150.066.669532
TAGGGAT20900.066.5324254
AAGAGGG94450.066.525142
GTAGGGA19850.066.4898153
CGAAGGG23900.066.070032
TAAGGGA26350.066.009033
AAGGGTA19050.065.817944
Produced by FastQC (version 0.11.5)