FastQCFastQC Report
Mon 27 Feb 2023
SRR3127455.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127455.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610051
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251000.9616670325599002No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43360.16612702203903296No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTG26490.1014922696912819Illumina PCR Primer Index 8 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85100.085.663671
TAGACGG2700.078.437471
CGTAGGG7250.075.892332
ACGGGTA3150.074.596464
ATAGCGG5900.074.1832351
AGGGATG44950.072.244885
ATAGGGA25950.071.534983
TAGGGCA14100.070.66034
TAGCGGG17300.069.589522
ACGGGAT9100.068.686124
AGTAAGG15500.067.709251
AGGGTAC10450.067.458035
CTACGAC9200.067.42875
GAGGGAT37950.067.366794
AGGGCAT25500.066.346965
AGTAGGG41750.066.232452
AAGAGGG95250.065.665412
TAGGGTC6800.065.6558464
GGATGGC38050.065.4605947
TAGAGGG43900.065.024082
Produced by FastQC (version 0.11.5)