FastQCFastQC Report
Mon 27 Feb 2023
SRR3127454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880413
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325961.1316432747665006No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41230.14313919566395514No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31430.10911629686437327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116750.085.298971
CGTAGGG8000.074.623462
TAGGGCA17600.074.5166244
GAATAGG16500.072.380991
AGGGATG47850.071.7137765
TAAGGGA32200.070.3643653
GGTAAGG19250.068.635741
GAGGGAT45200.068.4304054
ATAAGGG45450.068.260852
AGGGTAC13800.068.1259465
ATAGGGC18150.068.1146853
AGGGTAA17000.068.021755
ATAGGGA29450.067.836793
ATGCGGG15900.067.701772
ACGGGTA1950.067.4970864
CGAAGGG26950.067.152722
TAGAGGG51950.067.04932
AGGGCAT29050.066.9909065
TAAGGGT12800.066.837943
AAGGGAC29100.066.391194
Produced by FastQC (version 0.11.5)