FastQCFastQC Report
Mon 27 Feb 2023
SRR3127453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2509814
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262581.0462129863009768No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36890.1469830035213765No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG31970.1273799572398592Illumina PCR Primer Index 5 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28050.11176126995865031No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC27050.10777691095834192Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG26390.1051472340181384Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88350.085.901181
TAGGGCA15100.072.740594
AGGGATG41750.071.812765
ATAAGGG37400.071.5936662
TAAGGGA25850.070.93933
CGTAGGG6850.070.883392
GAATAGG15200.070.442041
AGGGTAA12350.070.395065
GAGGGAT39550.069.8472064
AGTAGGG44300.069.48762
CGAAGGG25350.069.363142
GGTAAGG17250.069.180081
AAGGGTA19050.069.041014
TAGAGGG43800.068.666422
ATAACGG2550.068.440191
AAGGGAC24850.067.9132464
ATAGGGA23300.067.575373
AGGGCAT24800.067.458355
TAGCGGG13650.066.998792
TAGGGAT20950.066.829824
Produced by FastQC (version 0.11.5)