FastQCFastQC Report
Mon 27 Feb 2023
SRR3127452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2556802
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254640.9959316364740015No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36590.143108461273106No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG33870.13247017172233125Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC28090.1098638064269349Illumina PCR Primer Index 5 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27920.10919891333001147No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG27760.10857313159173061Illumina PCR Primer Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88900.086.3128741
AGGGTAA12350.074.203845
GAATAGG16300.072.489631
TAGGGCA15650.072.070184
AGGGATG41550.072.048875
CGTAGGG6150.071.127972
ATAGGGA22150.071.077123
GAGGGAT38300.070.0641864
AGGGAAT30850.069.9223865
ATAACGG3100.069.8531341
TAGCGGG16600.069.70432
ATAAGGG38250.068.4946062
CGAAGGG25550.068.483292
TATAGGG18400.068.253672
ATGCGGG13750.068.0742652
AAGGGTA17400.068.062834
AGTAGGG46050.067.822072
ACGGGTA2300.067.428714
ATAGGGC17200.067.2148743
TAAGGGA25050.067.163613
Produced by FastQC (version 0.11.5)