FastQCFastQC Report
Mon 27 Feb 2023
SRR3127446.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127446.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2069198
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228781.1056457622711795No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42350.2046686687305903No Hit
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG28340.136961276784532RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC23420.11318394856364639RNA PCR Primer, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTG23080.1115407998654551RNA PCR Primer, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68700.084.1928561
AGTAAGG12050.074.597871
ATAGCGG4300.074.425291
AGGGATG36350.073.304155
CGAAGGG21150.072.046032
TAGGGCA12550.071.521994
CGTAGGG6000.071.32892
AGGGAAT22150.070.227015
TAAGGGA18450.069.537043
ATAGGGC12650.069.47063
GGTAAGG12450.069.177021
AGTAGGG35700.069.03012
ATGCGGG9200.069.011482
CTACGAC8800.068.890135
TAGCGGG12300.068.44212
GGATGGC32100.068.07667
GAGGGAT27350.067.871864
AGACGGG13100.067.852512
TATAGGG15350.067.711012
ATAGGGA22350.067.2856453
Produced by FastQC (version 0.11.5)