FastQCFastQC Report
Mon 27 Feb 2023
SRR3127445.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127445.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389457
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270081.1302986410720093No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26810.11220122396008801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85700.083.002731
ATAGCGG5150.073.945551
CGTAGGG7800.071.11152
ATAGGGC11950.070.803893
AATGCGG6300.069.4027561
GAATAGG14950.068.871131
TAGGGTA7850.068.862174
TAGAGGG46500.068.739832
AGGGTAT15450.068.4552155
TAGCGGG12800.067.938242
TATAGGG23200.067.67232
AGGGCAT24000.067.3751455
GTACGGG6350.066.622552
CGAAGGG23800.066.163792
AGGGATG34850.065.956645
ATAGGGA25250.065.901363
AAGAGGG85900.065.884892
AGTAAGG15450.065.7293851
GGTAAGG14750.065.661311
GATTAGG8850.065.342561
Produced by FastQC (version 0.11.5)