FastQCFastQC Report
Mon 27 Feb 2023
SRR3127444.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127444.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070461
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225831.0907232737057109No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG26610.1285221020825797No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG23330.11268021952598962No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC23300.11253532425870375No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22610.1092027331111284No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74500.085.070641
TAGGGCA10100.073.713374
AGGGATG30300.071.189335
ATAGGGA19750.070.621213
ATAGGGC12000.070.288193
TAGAGGG37950.069.780672
AAGAGGG70850.069.0348052
ACGGGAT8600.068.489214
AGGGCAT19850.068.419865
AGTAGGG31100.068.332412
CGTAGGG5050.068.114892
GAATAGG11500.068.068621
TAAGGGA17800.067.5041963
ATAGCGG4200.067.365681
CGAAGGG19550.067.246252
AGTAAGG13150.067.0583041
GGTAAGG13750.066.532791
GTAAGGG32550.066.012242
AGACGGG11250.065.759382
AGGGTAT12050.065.5189745
Produced by FastQC (version 0.11.5)