FastQCFastQC Report
Mon 27 Feb 2023
SRR3127443.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127443.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111692
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218541.0349047114825458No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG28050.1328318713145667No Hit
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTG23860.11298996255135693No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC23110.10943830823813322No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22950.1086806219846455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72300.084.044051
ATAGCGG4450.071.923031
ACGGGTA2300.071.517274
CGGGTAT1600.070.49565
AGTAGGG32200.069.948222
AGGGATG29500.069.7786945
AGGGCAT19400.069.768835
TAGGGCA10100.068.866984
TAGGGCG3700.068.590314
AGTACGG2000.068.247551
GAATAGG12900.068.22931
TAGAGGG36800.067.849042
GGTAAGG13000.067.704471
TAAGGGA18000.067.362463
TATAGGG17250.067.329422
TAGCGGG10600.066.983542
AGTAAGG12500.066.647261
AAGAGGG77250.065.9214862
CGAAGGG21000.065.830182
ATAGGGA18850.065.57153
Produced by FastQC (version 0.11.5)