FastQCFastQC Report
Mon 27 Feb 2023
SRR3127440.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127440.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645818
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83051.285966015193135No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45040.6974101062528452No Hit
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG29890.4628238915607803TruSeq Adapter, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC28280.43789426742518794Illumina PCR Primer Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG26220.4059967359225045TruSeq Adapter, Index 14 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTC25600.3963965079945124No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA12270.1899916075426823No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT9980.1545327011634857Illumina PCR Primer Index 3 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT9350.14477763084955825Illumina PCR Primer Index 3 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCATT8900.13780972348246717No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8430.13053213134350544No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCATTGCGTCTCG8350.1292933922560226No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT7850.12155127295925477No Hit
GAATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTT7700.11922863717022444TruSeq Adapter, Index 14 (95% over 21bp)
TAGACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCT7500.1161317894515173No Hit
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG7220.11179620264532733Illumina PCR Primer Index 3 (95% over 22bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATG6710.10389924096262414No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG6610.10235081710327058No Hit
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT6520.10095723562985237TruSeq Adapter, Index 14 (95% over 23bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCATTGCGTCTC6460.10002818131424024No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGG201.8328627E-594.045622
CGAACGG156.896021E-494.01649594
ACGGGTA450.093.994664
TATCGAG156.902392E-493.994669
GTAGCGG750.087.871221
GCTACGA7550.087.7698444
CGCTACG7700.087.280763
CGTTTTT36150.086.8555151
TAAGCGG1100.085.5888441
TCCGCTA8150.084.328641
TAGCGGG2900.084.3167652
AGGGTAC2700.083.5508045
TCGCGGG850.082.981442
TACGACC8100.081.8101656
GTAGGGC2500.080.835413
CTACGAC8200.080.8124855
AAGGGCA7100.080.7559744
ATTACGG353.8200596E-880.698061
GGATGGC14500.080.0575267
CGACCAA8050.079.983038
Produced by FastQC (version 0.11.5)