FastQCFastQC Report
Mon 27 Feb 2023
SRR3127439.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127439.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771564
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363441.3113173644916734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116150.085.4988251
TAGGGCA17300.079.0634164
ATAGGGC16950.076.8123253
CGTAGGG7500.072.697192
AGTAGGG46250.070.224262
ATAGGGA24550.070.072953
TAGAGGG52100.069.73692
ATAGAGG20050.068.478451
TATAGGG24650.066.7378162
AGGGCAT28050.066.357655
TAGGGTA10350.066.304224
AATAGGG41400.066.303032
AGGGTAC13200.065.875895
GTAGGGC16450.065.7177663
TAAGGGA26400.065.162543
GAATAGG16650.064.952971
AGAGGGC42450.064.773753
AAGGGCA48600.064.7021264
ATAAGGG43200.064.628422
GTAGGGT10000.064.393413
Produced by FastQC (version 0.11.5)