FastQCFastQC Report
Mon 27 Feb 2023
SRR3127438.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127438.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401727
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309911.2903631428551205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97900.085.4706651
TAGGGCA12450.074.6039054
ATAGGGC13300.072.671973
CGTAGGG6600.070.722232
AGTAGGG40250.070.634382
GAATAGG16250.069.953571
TAGAGGG45650.069.405382
ATAGGGA20850.068.743583
ATAGAGG15850.068.743061
TAAGAGG18400.068.188321
TATAGGG19650.067.423062
AGGGATG35200.067.0185245
AATAGGG36450.067.003412
TAAGGGA21200.066.7190863
ATAAGGG34550.066.457742
AAGAGGG91450.066.4559562
AAGGGCA38300.065.244194
AAGGGTA17450.064.84564
TAGGGAT14050.064.765794
AGAGGGC33450.064.414673
Produced by FastQC (version 0.11.5)