FastQCFastQC Report
Mon 27 Feb 2023
SRR3127437.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127437.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435114
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297741.2226942968583812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97100.084.61361
ATAGGGC13950.074.7895053
TAGGGCA13500.073.453174
CGTAGGG6900.072.247362
ATAGGGA20000.070.964133
AGTAGGG38100.070.234822
TAGAGGG46800.069.6390152
GAATAGG12650.069.561061
ATAGCGG5200.068.774171
AGAGGGC37400.068.232323
TAGGGTA8000.066.969464
TATAGGG20950.066.67112
AAGAGGG95200.065.8012
AGATAGG13750.065.365081
ATAAGGG35350.065.321672
TAGGGAT13700.065.1770864
AGGGATG33600.065.039265
AGGGCAT20700.064.931825
AATAGGG33200.064.735622
ATAGAGG18400.064.702021
Produced by FastQC (version 0.11.5)