FastQCFastQC Report
Mon 27 Feb 2023
SRR3127435.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127435.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355081
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318521.352480020857032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT103950.087.40471
CGTAGGG6250.076.983692
ATAGGGA18200.073.6080863
AGGGATG37200.072.635665
TAGGGTA6850.072.3064964
GTACGGG5550.072.244452
GGTAAGG14100.070.630441
TAGGGAT14400.070.101914
AGTAGGG43350.069.315272
TAGAGGG43650.068.406612
ATAGCGG6000.067.651241
AGAGGGC36750.067.259333
TAACGGG6550.066.976162
TAGGGCG3900.066.523744
TAGGGCA9950.066.3717964
GATAGGG32050.065.936912
ATAGGGC11700.065.717393
TAAGGGA19550.065.388463
AAGAGGG85650.065.318462
GTAGGGT9850.065.130033
Produced by FastQC (version 0.11.5)