FastQCFastQC Report
Mon 27 Feb 2023
SRR3127434.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127434.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388301
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308761.2928018704510027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98150.087.0177841
ACGGGTA2250.075.193034
CGTAGGG6650.074.25652
ATAGGGA21100.073.054853
GTACGGG6150.071.117212
GAATAGG14050.071.003741
TAGAGGG45150.070.6217962
AGGGATG37400.070.116495
AGTAGGG41850.069.448022
AGAGGGC36350.068.004713
TAGGGCA10550.067.7093664
ATAGAGG17750.067.07241
TAAGGGA22100.066.3467943
GTAGGGA17500.066.3313
ATAGGGC11850.066.2301563
AGTTAGG6750.066.227981
AGATAGG16100.066.054791
TAGGGCG4850.065.89084
GATAGGG34550.065.88162
AAGAGGG89300.065.830242
Produced by FastQC (version 0.11.5)