FastQCFastQC Report
Mon 27 Feb 2023
SRR3127433.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127433.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2737561
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355791.2996605372446495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT113600.086.790891
ATAGGGC14100.074.67583
CGTAGGG9300.073.793962
TAGGGCA14850.072.170444
TAGGGTA9450.071.1303944
GGTAAGG17600.070.79251
TAGAGGG52750.070.040792
AGTAGGG46950.069.682632
AGGGTAC12050.069.4358755
GAATAGG16200.069.3644561
AGGGATG43500.069.049825
GAGGGAT34150.068.271894
TATAGGG22450.068.257782
TAAGGGA24350.068.1438453
ATAGCGG6900.067.4590451
AGAGGGC39250.066.1074453
AGGGTAT14800.065.426925
ATGCGGG14600.065.357312
AAGAGGG98200.065.147482
CGAAGGG28800.065.122542
Produced by FastQC (version 0.11.5)