FastQCFastQC Report
Mon 27 Feb 2023
SRR3127432.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127432.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374692
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297191.2514886141023762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90400.086.320011
CGTAGGG6400.075.099342
GGTAAGG13100.071.2649151
TAGGGCA10900.070.897884
AGGGATG35800.070.753775
ATAGGGC12650.069.284863
TAGAGGG45250.068.72922
AGTAGGG39600.068.063892
TAGCGGG14550.068.009912
ATAAGGG34950.067.007772
GAATAGG12950.066.628991
ATGCGGG13300.066.6073462
AGGGAAT23300.066.558255
GTAGGGA15150.066.249543
TAGGGTA7200.066.100514
CGAAGGG24800.065.741612
GTAGGGT7750.065.665613
AATGCGG6250.065.632831
AAGAGGG85350.065.533472
GAGGGAT27850.065.478944
Produced by FastQC (version 0.11.5)