FastQCFastQC Report
Mon 27 Feb 2023
SRR3127431.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127431.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419728
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292171.2074497629485628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91400.086.971831
TAGGGCA12100.075.3499454
ATAGGGC13250.074.130513
CGTAGGG7900.070.840672
GAATAGG14400.067.982331
TAGAGGG46700.067.9750752
AGGGATG35750.067.307045
AGTAAGG15900.066.6009751
AGGGTAC9600.066.578535
AATAGGG35100.066.456482
GAGGGAT29000.066.443484
TATAGGG19250.065.96232
GGTAAGG14500.065.8905641
AGTAGGG42150.065.605842
TAAGCGG7750.065.586921
ATAGGGA16700.065.570123
ATAGAGG18950.065.5676961
AAGAGGG86500.065.296462
AAGGGTA15650.065.164634
ATAAGGG33950.064.690412
Produced by FastQC (version 0.11.5)