FastQCFastQC Report
Mon 27 Feb 2023
SRR3127430.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127430.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518845
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT656232.605281388890543No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT32040.12720115767345747No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT29930.11882430240844513No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26680.10592156325617495No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT25850.10262640218036442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT214000.088.9457551
TAGGGCA12300.074.1374
ATAGGGC14200.073.154723
AGGGATG39750.069.4127665
AGTAGGG38300.068.849772
ATAGGGA23100.067.963053
AATAGGG36450.067.0570142
TAGCGGG15500.067.019162
AGAGGGC37000.066.441343
TAGAGGG45700.066.135282
GAGGGAT31050.065.700174
GATAGGG37650.065.6688162
AGGGCAT22200.065.2132955
GAATAGG15050.064.690371
GTAGGGT10050.064.543343
TAGGGTC7750.064.289874
GGTAAGG15600.064.21861
AGTAAGG17150.064.173771
ATAAGGG35550.063.8625032
AAGAGGG88400.063.806862
Produced by FastQC (version 0.11.5)