FastQCFastQC Report
Mon 27 Feb 2023
SRR3127429.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127429.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2198473
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT557522.53594199246477No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT40120.18249030122271231No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT38930.17707745330508948No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT34270.15588092280414634No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC24950.11348786180226003Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG23490.10684688872685724No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22830.10384480500783955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT182700.089.13291
ATAGGGC11200.074.917593
AGGGATG32900.074.27525
AGTAGGG33400.073.1080252
TAGGGCA10850.071.6862644
TAGAGGG37250.069.601432
AGTAAGG13600.069.0071641
AGGGTAC9750.068.9235845
TAGGGTA7850.067.255864
ATGCGGG10150.066.877222
GTAGGGT7700.066.729453
ATAGGGT8800.066.4233553
GGATAGG13350.065.707011
GGTAAGG11700.065.702481
GAGGGAT28450.065.613724
TAGCGGG12350.065.269592
AAGAGGG73850.065.235222
ATAGAGG16050.065.231581
AGGGAAT24400.064.904755
GATAGGG31450.064.30112
Produced by FastQC (version 0.11.5)