FastQCFastQC Report
Mon 27 Feb 2023
SRR3127428.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127428.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2230739
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542402.4314812266248986No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT41370.18545423736259598No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT38960.17465064267939906No Hit
CGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT36050.1616056383108916No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC26230.11758435209139213Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG24680.11063598206692939No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22500.10086343583897535No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT174450.089.033631
ATAGGGC13300.074.55883
ATAGCGG4900.073.000961
TAGGGCA11550.072.427944
AGTAGGG36450.069.676412
AGGGATG32800.069.6354755
ATAGGGA18750.067.424723
ATAGAGG15350.067.150121
GTAGGGT8350.066.977453
AGGGCAT18500.066.811645
GATAGGG32900.066.616152
AGGGTAC9600.066.578775
TAGAGGG39800.066.293242
AGAGGGC32550.065.983193
AAGAGGG73350.065.658252
AGGGAAT22300.065.12115
AATAGGG30750.065.002992
GGTAAGG11900.064.864631
AAGGGTA13050.064.102894
GAGGGAT26550.063.9014664
Produced by FastQC (version 0.11.5)