FastQCFastQC Report
Mon 27 Feb 2023
SRR3127427.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127427.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340765
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63731.8702038061420627No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC17470.5126700218625739No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG13550.3976347336140742No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11240.329846081610494No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC10960.3216292753070298No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG10510.30842369374789075No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACGTCG6020.17666133552448168No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG4740.13909879242293077No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC4550.1335231024312943No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT4340.1273604977036961No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT4240.12442592402388743No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC3750.11004651299282497No Hit
GGGAAGGTGGACCAGAATGAAAACTGTAAATATTTTTTTAACCTAATATC3690.10828576878493977No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG3560.10447082300118851No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG156.8873586E-494.036551
GGCAACG156.895401E-494.008947
TAGGGTA1050.089.532334
CGTTTTT29700.088.4956741
AATGCGG650.086.802981
GTGTAGG600.086.200171
TAGCGGG1700.085.714042
AGGGATG7350.083.776695
AGGGTAT1350.083.5635155
CGCTACG3250.082.438623
GCGTAGG401.029548E-982.2819751
CAGGGTA800.082.257834
ATAGCGG750.081.4983441
AGGGCAT2150.080.8914265
TCCGCTA3550.080.791971
AGGGAAT7400.080.6698465
GGATACG353.853893E-880.57918
CGTAGGG700.080.57912
TAGGGCG700.080.57914
GTGAAGG1700.080.207651
Produced by FastQC (version 0.11.5)