FastQCFastQC Report
Mon 27 Feb 2023
SRR3127426.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127426.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312103
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49001.5699945210395287No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG15690.5027186537777593No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15410.49374725651467627No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG15200.48701870856736396No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC14720.4716391704020788No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG9090.29125000400508805No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9050.28996837582464763No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACGTCG7700.24671342473478308No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT5750.18423405093831202No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC5680.1819912016225413No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT5590.17910753821655032No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT4640.14866886893109005No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA4570.1464260196153193No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC4310.1380954364424565No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG4300.1377750293973464No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG4130.13232810963047456No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG3820.1223954912320612No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTA3610.11566694328474895No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT3460.11086083760809733No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG3280.1050935107961154No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT3260.10445269670589516No Hit
GACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTC3220.10317106852545474No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC3210.10285066148034462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGG500.094.245131
CATGGGA201.8136441E-594.233
AATGCGG1000.089.5328751
TAACGGG950.089.270532
CGTTTTT22350.089.184991
AGGGAAT6750.087.722575
TAGGGCG650.086.9815444
AGGGCGA1200.086.15615
AGGGTAC1050.085.037185
TCCGCTA3200.083.937071
ACTAGGG1350.083.762
CGCTACG3150.083.763
AAGGGAT7400.083.406284
GTAAAGG1700.083.157481
ATGAGGG2900.082.8574142
AGGGTAT1250.082.7098545
ATAGCGG401.0113581E-982.464491
TAAGCGG800.082.464491
CACGGGT401.0131771E-982.451253
AGCGGGA1600.082.451253
Produced by FastQC (version 0.11.5)