FastQCFastQC Report
Mon 27 Feb 2023
SRR3127425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322598
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48111.4913297664585645No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACG22200.6881629768318465No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG16610.5148822993322959No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16380.5077526829056597No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC15610.4838839670425731No Hit
GCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG14690.4553655013360281No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA11110.34439147173882045No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8620.26720562433741063No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGAATCACGTCG8390.2600760079107744No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC6020.18660996038413133No Hit
CGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT6010.1862999770612341No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATG5920.18351012715515905No Hit
GAATCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT5780.17917036063459785No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG5460.16925089430188656No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5430.1683209443331949No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAATCACGTC4960.15375172815702515No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG4500.13949249530375266No Hit
AGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT4260.13205289555421917No Hit
AAAGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT4020.12461329580468572No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCC3840.1190335959925356No Hit
ACTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG3810.11810364602384393No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC3690.11438384614907716No Hit
GACACACCCACACCTGTCTCTTATACACATCTGACGCGAATCACGTCGTA3320.10291446320187973No Hit
GGCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCT3230.10012461329580469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG301.31312845E-894.1005551
CTAACGG156.868418E-494.1005551
GCGTAGG301.31312845E-894.1005551
GGTACGT156.8981264E-493.998457
GGTACGC201.836034E-593.998457
TAGGGCG600.093.998454
CCACATG254.9135997E-793.998458
TAACGGG950.089.092592
CGTTTTT23550.088.9060441
GAATAGG1300.086.862051
GGGTACG600.086.1652456
ATGGGTA550.085.453134
ATAGCGG500.084.6905061
AACGGGT500.084.59863
TATAGGG1900.084.1432
GGTAAGG1800.083.644941
AGCGGGA1750.083.2557753
AAGGGTC1250.082.718634
AGGGATG5900.082.049495
AGGGAAT8500.081.833945
Produced by FastQC (version 0.11.5)