FastQCFastQC Report
Mon 27 Feb 2023
SRR3127424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459226
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395951.1446202127296685No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC70560.2039762652107726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT133450.083.9968261
CGTAGGG10050.077.177842
AGGGATG62050.073.4860155
ATAGCGG6850.072.7596361
ACGGGTA3450.070.853134
TAAGGGA32950.070.3341453
GTAGGGT12400.070.133383
AGGGCAT32050.069.8157965
ATAGGGA32700.068.571773
AGAGGGC49200.068.4104843
AGTAGGG59050.068.303372
TAGAGGG61050.068.144742
AAGGGTA26200.068.000594
TAACGGG13000.067.981262
TAGGGCA17950.067.304394
ATAGGGC21450.067.279973
AGGGTAC19100.066.697675
GAGGGAT51300.066.526394
AAGGGCA55000.066.4099354
AGTAAGG21800.066.215111
Produced by FastQC (version 0.11.5)