FastQCFastQC Report
Mon 27 Feb 2023
SRR3127423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961726
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323231.0913568642068847No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61110.2063323886139366No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG35810.12090922657936622No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC34890.11780292977810912No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG32280.10899050080932536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111850.086.359781
CGTAGGG8900.076.7933042
ACGGGTA4350.075.849754
AGGGATG53950.075.172275
ATAGGGA25900.071.885733
GTACGGG7500.071.645512
AGAGGGC40800.071.280443
GTAGGGT9450.070.827493
ATAGGGC17450.070.23013
AGGGCAT26300.070.043635
AATGCGG5750.069.721811
TAGGGCG3200.069.229834
AGTAGGG47050.069.225132
GGTAAGG20000.068.153071
TAGAGGG51850.068.089232
CGAAGGG30600.067.9301452
AAGGGCA44500.067.789954
ATGCGGG11900.067.73212
TAAGGGA26750.067.663243
AAGAGGG104550.067.580752
Produced by FastQC (version 0.11.5)