FastQCFastQC Report
Mon 27 Feb 2023
SRR3127422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3058133
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313911.0264759577166853No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61100.19979510374467038No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG36770.12023675883292191No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC35640.11654169390278317No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG33660.11006715535262855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105350.085.0546341
CGTAGGG8750.074.7079242
AGGGATG52800.072.0037165
ATAGCGG6500.071.678261
ACGGGTA3350.071.5427554
TAGGGCG3300.071.202684
TAGGGTA10200.070.9513854
ATAGGGC19950.070.902883
TAGGGCA16650.070.843474
AGAGGGC43850.070.731793
TAGCGGG18050.070.08662
TAGAGGG53100.069.346922
TAAGGGA27450.069.335073
AAGGGTA21850.069.03894
AGGGTAC14250.068.9241945
GTAGGGT10350.068.560963
AGGGCAT29550.068.383495
CTACGAC11650.068.171235
TAGGGTC7800.068.080724
GAATAGG19000.067.867611
Produced by FastQC (version 0.11.5)