FastQCFastQC Report
Mon 27 Feb 2023
SRR3127421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3687312
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508991.3803822405047361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT171250.086.478091
CGTAGGG11350.073.3022842
AGGGATG63350.070.191515
ATAGGGC21000.069.163873
AGTAGGG62100.068.8037952
ATAGGGA37200.068.3587953
ACGGGAT13150.068.27284
TAGAGGG73950.068.266272
TAAGGGA31850.067.887293
TAGGGCA20300.067.149384
ATAACGG3700.066.094541
AAGAGGG135700.065.709412
GAGGGAT53950.065.606014
GTACGGG8700.065.3741762
ATGCGGG20950.064.841632
GATAGGG55500.064.62072
ATAAGGG53450.063.7573742
GGTAAGG23550.063.503871
AAGGGCA59150.063.4939164
TATAGGG29100.063.4804042
Produced by FastQC (version 0.11.5)