FastQCFastQC Report
Mon 27 Feb 2023
SRR3127420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147021
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411321.30701383943736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT134950.086.702271
CGTAGGG9250.074.359412
ATAGGGC18300.072.3402563
ACGGGAT8950.071.588074
AGTAGGG51700.071.168082
AGGGATG47350.071.062435
TAGGGCA16250.070.739394
GGTAAGG20200.069.778471
GTAGGGC13900.069.141723
AAGAGGG117000.067.2040252
AGAGGGC45050.065.464273
ATAGGGA26850.065.2714843
TAGAGGG58400.065.0200042
GAGGGAT39300.064.733044
ATAGCGG7000.064.6509251
TAGGGAT23250.064.436064
GAATAGG18150.064.413461
TAAGGGA26050.064.382393
TAGACGG4700.064.192411
TACACGG2350.064.192411
Produced by FastQC (version 0.11.5)